Knopov, Victor,Witte, Richard P.,Karmali, Priya,Lee, Robin,Webb, David,Akopian, Violetta
申请号：
EP19205352
公开号：
EP3673898A1
申请日:
2012.11.02
申请国别(地区):
EP
年份:
2020
代理人:
摘要：
What is described is a method for preparing a liposome that efficiently encapsulates a negatively charged therapeutic polymer, e.g., siRNA. The process involves preparing a lipid mixture comprising a cationic lipid in a water miscible organic solvent such as ethanol, and injecting this solution to the polymer dissolved in water to a final concentration of 35% ethanol in water to produce nanoparticles having a mean size of 50 to 150 nm and a final charge ratio of drug:lipid is 1 :2.5.
