Victor Knopov,Richard P. Witte,Priya Karmali,Robin Lee,David Webb,Violetta Akopian
申请号：
US13669078
公开号：
US20130115274A1
申请日:
2012.11.05
申请国别(地区):
US
年份:
2013
代理人:
摘要：
What is described is a method for preparing a liposome that efficiently encapsulates a negatively charged therapeutic polymer, e.g., siRNA. The process involves preparing a lipid mixture comprising a cationic lipid in a water miscible organic solvent, such as ethanol, at a concentration of 2.3 mg/ml, and adding this solution to the polymer dissolved in water to a final concentration of 35% ethanol in water. The final charge ratio of drug:lipid is 1:2.5. The resulting nanoparticles have a mean size of 50 to 150 nm.
