Timothy Tree;Robert J. Deans;Samantha Stubblefield;James Reading
发明人:
Robert J. Deans,James Reading,Samantha Stubblefield,Timothy Tree
申请号：
US15767085
公开号：
US20190358258A1
申请日:
2015.10.09
申请国别(地区):
US
年份:
2019
代理人:
摘要：
The invention is generally directed to a method of enhancing proliferation of T regulatory cells (Tregs) in vitro, comprising contacting Tregs with cells (I), or conditioned medium from the cells, in the presence of one or more Treg stimulation agents. The Treg stimulation agent(s) is present in an amount and for a time effective to stimulate proliferation of the Tregs. The cells (I) are present in an amount and for a time effective to enhance proliferation of the Tregs. The cells (I) are non-embryonic stem, non-germ cells characterized by one or more of the following: extended replication in culture and express markers of extended replication, express markers of pluripotentiality, and have broad differentiation potential, are not tumorigenic or transformed, and have a normal karyotype. The invention is also directed to methods for immune modulation using the proliferated Tregs, cell banks, drug discovery methods, populations, and compositions of the proliferated Tregs.
