SJOEGREN, ANITA,KILMARE, EVA KARIN,ENERBAECK, SVEN,ERIKSSON, PETER
申请号：
CA2524929
公开号：
CA2524929C
申请日:
2004.05.10
申请国别(地区):
CA
年份:
2015
代理人:
摘要：
An improved method for vitrification of biological cells, especiallyblastocyst-derived stem cells (BS cells). The method is very mild for thecells that remain viable after they have been thawed. The method comprises, i)transfer of the cells to a first solution (solution A), ii) optionallyincubation of the cells in the first solution, iii) transfer the cellsobtained in step i) or ii) to a second solution (solution B), iv) optionallyincubation of the cells in the second solution, v) transfer of the cellsobtained from step iii) or iv) into one or more closed straws with dimensionsthat allow a volume of at least 20 ~l to be contained in them vi) sealing theone or more closed straws, and vii) vitrification of the one or more closedstraws. An important feature of the present invention is the use of closedstraw and that relatively large volumes can be efficiently vitrified andsubsequently thawed.
