The present invention is about the method of fertilization and the determination and identification of the gender of the subject in aviation. More specifically, the invention provides a non-invasive method using cross gene avian animals, including at least one indicator gene, specifically, RFP, In at least one Z or W chromosome, the transgenetic avian animal of the invention is used to identify and select embryos from uncultivated eggs.La presente invencion se refiere a metodos de fertilizacion y determinacion e identificacion del genero en sujetos aviares. Mas especificamente, la invencion proporciona metodos no invasivos que usan animales aviares transgenicos que comprenden al menos un gen indicador, especificamente, RFP, integrado en al menos un cromosoma de genero Z o W. Los animales aviares transgenicos de la invencion se usan para la determinacion del genero y la seleccion de embriones en huevos aviarios sin eclosionar
