Institute of Crop Sciences; Chinese Academy of Agricultural Sciences
发明人:
申请号：
EP19170389.1
公开号：
EP3561079A1
申请日:
2019.04.19
申请国别(地区):
EP
年份:
2019
代理人:
摘要：
The present invention provides a soybean anti-pod-shattering major QTLqPD08-1, and a mapping method and application thereof； a soybean anti-pod-shattering gene and an application thereof, which belongs to the field of QTL mapping； map-based cloning； molecular-marker development and conventional breeding； and a genetic transformation application of a pod-shattering gene. The soybean anti-pod-shattering major QTLqPD08-1 is mapped on the chromosome 8 of soybean at a physical position between 35560353-38482164. For the method for mapping the soybean anti-pod-shattering major QTL, a SLAF marker is screened at the whole genome level of the soybean by utilizing a SLAF-seq sequencing technology, so as to explore the QTLs related to pod shattering from this population. By using a material of a RIL7 population which has pod-shattering soybean and anti-pod-shattering soybean as the parents, a high-density genetic linkage map covering the whole genome of soybean is constructed, and QTL mapping of the anti-pod-shattering trait is carried out on this population to obtain QTLs related to anti-pod-shattering. In addition to this, the construction of the high-density genetic linkage map and the identification of the novel QTLs related to anti-pod-shattering specific to this population provide a reference for QTL mapping of soybean, map-based cloning, transgenic breeding of a pod-shattering gene, molecular-marker development and breeding applications, and meanwhile the soybean anti-pod-shattering gene provides a basis for selection of anti-pod-shattering varieties of soybean.
