An alignment module for center point of motion of minimally invasive surgical instrument comprises a connecting piece, a buckling piece and an alignment piece. The connecting piece, the buckling piece and the alignment piece are combined together by buckling. The alignment piece is configured with an instrument penetrating hole that is provided for a surgical instrument inserted therein. A projector, which is configured on a plane coplanar to the center line of the instrument penetrating hole, emits a light beam that forms an angle relative to the center line of the instrument penetrating hole.本發明係為一種微創手術器械的運動中心點之對位模組，係包含：一連結件、一卡扣件及一對位件，該連結件、卡扣件及對位件之間係藉由卡扣方式相互結合，該對位件係設有一器械穿設孔，該器械穿設孔係提供一手術器械插設其中，且於該器械穿設孔的中心線之同一平面設有一投影器，該投影器所發射的光束係與該器械穿設孔之中心線夾一角度設置。
