The present invention relates to a compound of Antrodia camphorata used to inhibit HBV, in particular to an extract, 4-hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone which is isolated from Antrodia camphorata, and its use in inhibiting HBV effectively. The cyclohexenone compound according to the present invention showed cytotoxicity on HBV-secreting human hepatoma cell line HepG2 2.2.15, decreased synthesis of HBV particles, further inhibited synthesis of HbsAg and HbeAg effectively to achieve the goal of HBV inhibition.本發明係關於一種用於抑制B型肝炎病毒之牛樟芝環己烯酮化合物，尤其是關於一種由牛樟芝萃取物中所分離出之4－羥基－2,3－二甲氧基－6－甲基－5(3,7,11－三甲基－2,6,10－十二碳三烯)－2－環己烯酮(4－hydroxy－2,3－dimethoxy－6－methy－5[3,7,11－trimethyl－do deca－2,6,10－trienyl]－cyclohex－2－enone)並可有效抑制B型肝炎病毒。本發明中，牛樟芝環己烯酮化合物對於能分泌B型肝炎病毒之人類肝癌細胞株HepG2 2.2.15係具細胞毒性，並可降低B型肝炎病毒顆粒之生成量，且可有效抑制B型肝炎表面抗原(HbsAg)以及B型肝炎外套膜抗原(HbeAg)，藉以達到抑制B型肝炎病毒之目的。
