INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM);INSTITUT PASTEUR;FONDATION IMAGINE;ASSISTANCE PUBLIQUE - HOPITAUX DE PARIS;UNIVERSITE PARIS DESCARTES
发明人:
EBERL, GERARD,BIKARD, DAVID,SCHNUPF, PAMELA,CERF BENSUSSAN, NADINE,GABORIAU-ROUTHIAU, VALERIE,SANSONETTI, PHILIPPE
申请号：
CA2971933
公开号：
CA2971933A1
申请日:
2015.12.23
申请国别(地区):
CA
年份:
2016
代理人:
摘要：
The present invention relates to an in vitro method of culturing a segmented filamentous bacterium strain, comprising co-culturing said segmented filamentous bacterium strain with a eukaryotic host cell, wherein the culture is performed at an O2 level inferior to 5% in a rich tissue culture liquid medium containing bacterial medium components including iron. The present invention also relates to methods for genetically modifying a segmented filamentous bacterium strain comprising a step a culturing the strain in vitro.
