Compositions comprising modified recombinant polymerizing enzymes are provided, along with nucleic acid molecules encoding the modified polymerizing enzymes. In some aspects, methods of using such polymerizing enzymes to synthesize a nucleic acid molecule or to sequence a nucleic acid template are provided.本發明提供包含經修飾重組聚合酶之組合物以及編碼該等經修飾聚合酶之核酸分子。在一些態樣中,提供使用此類聚合酶合成核酸分子或定序核酸模板之方法。