Provided are nanoparticles, method of using and making thereof. The invention nanoparticle comprises a) an inner core comprising a non-cellular material； and b) an outer surface comprising a cellular membrane derived from a cell or a membrane derived from a virus. Medicament delivery systems or pharmaceutical compositions comprising the inventive nanoparticles are also provided. The present invention further provides immunogenic compositions comprising the inventive nanoparticles, and methods of use the inventive immunogenic compositions for eliciting an immune response and for treating or preventing disease or condition, such as neoplasm or cancer, or disease or conditions associated with cell membrane inserting toxin. Vaccines comprising the immunogenic composition comprising the nanoparticles of the present invention are also provided.本發明提供奈米粒子、其使用及製備方法。本發明奈米粒子包含a)包含非細胞物質之內部核心；及b)包含來源於細胞之細胞膜或來源於病毒之膜之外部表面。亦提供包含本發明奈米粒子之藥劑傳遞系統或醫藥組合物。本發明更提供包含本發明奈米粒子之免疫原性組合物、及使用本發明免疫原性組合物引發免疫反應及治療或預防疾病或病狀，諸如贅瘤或癌症或與細胞膜插入毒素相關之疾病或病狀的方法。亦提供包含含有本發明之奈米粒子之免疫原性組合物的疫苗。
