Federalnoe gosudarstvennoe byudzhetnoe nauchnoe uchrezhdenie "Tomskij natsionalnyj issledovatelskij meditsinskij tsentr Rossijskoj akademii nauk ("Tomskij NIMTS")
发明人:
Bragina Olga Dmitrievna (RU),Брагина Ольга Дмитриевна (RU),Chernov Vladimir Ivanovich (RU),Чернов Владимир Иванович (RU),Zelchan Roman Vladimirovich (RU),Зельчан Роман Владимирович (RU),Medvedeva Anna
申请号：
RU2019101464
公开号：
RU0002702294C1
申请日:
2019.01.18
申请国别(地区):
RU
年份:
2019
代理人:
摘要：
FIELD: medicine.SUBSTANCE: invention refers to medicine, namely to oncology and radionuclide diagnostics, and can be used for diagnosing operable breast cancer with Her2/neu overexpression. Injection form of the radiopharmaceutical is introduced on the basis of technetium-99m labeled recombinant address molecules DARPin9_29, which is prepared immediately before administration. For this purpose in aseptic conditions 1 ml of eluate 99mTcO4- 7 GBq is added by syringe into kit for preparation of tricarbonyl technetium and incubated at temperature of 100 °C for 30 minutes. After incubation, 1,000 mcl of technetium tricarbonyl is added to 334 mcl of DARPin9_29 at concentration of basic substance solution of 3.6 mg/l and incubated at temperature of 40 °C for 60 minutes. Further, the obtained compound is purified from protein impurities and non-technetium-bound DARPin9_29 molecules by means of cleaning columns. 500-MBq-produced preparation is diluted in 10 ml of physiologic saline and slowly introduced into the patient through a sterilizing filter. In 4 hours after administration of the preparation, a single-photon emission computed tomography is performed on a two-detector gamma camera. Obtained results are evaluated and the malignant tumor is diagnosed when visualizing the RFP fixation in the mammary gland tissues.EFFECT: technical result provides higher specificity, information value and accessibility of diagnosing operable breast cancer with Her2/neu overexpression.1 cl, 2 dwg, 2 exИзобретение относится к медицине, а именно к онкологии и лучевой радионуклидной диагностике, и может быть использовано для диагностики операбельного рака молочной железы с гиперэкспрессией Her2/neu. Вводят инъекционную форму радиофармпрепарата на основе меченных технецием-99m рекомбинантных адресных молекул DARPin9_29, который изготавливают непосредственно перед введением. Для чего в асептических условиях 1 мл элюата 99mTcO4- 7 ГБк с помощью шприца добавляют в набор для приготовления трикарбо
