The present invention relates to a built-in bio-sleeve and preparation method and use thereof. In order to provide a suitable built-in bio-sleeve material for dorsal nerve isolation surgery and penis enlargement surgery, a novel acellular allogeneic dermal matrix material is prepared in the present invention, which is manufactured into a built-in bio-sleeve for the treatment of premature ejaculation and short and small penis. The acellular allogeneic dermal matrix or the built-in bio-sleeve of the present invention is used for dorsal nerve isolation surgery, penis enlargement or lengthening surgery without cutting off the dorsal nerve, thereby avoiding the probability of psychological erectile dysfunction caused by the patient's worry about cutting off the dorsal nerve.
