Compositions, methods, and uses of recombinant recombinant IL-8 antibody, fragment thereof or single chain variable fragment (scFv) having high affinity to IL-8 to target tumor-expressed or endogenous IL-8 are presented. Preferably, the recombinant IL-8 antibody or scFv fragment includes a VHsegment comprising a first amino acid sequence selected from SEQ ID NO. 1-15, 31-32, and/or a VLsegment comprising a second amino acid sequence selected from SEQ ID NO. 16-30, 33-34. The recombinant IL-8 antibody or scFv fragment can be formulated as pharmaceutical compositions to administer to a patient having a tumor to reduce metastasis of the tumor, reduce immune suppression in the tumor microenvironment or reduce Th2 mediated immune response.本案提供對IL-8具有高親和力的重組IL-8抗體、其片段或單鏈可變片段(single chain variable fragment, scFv)之組合物、方法，以及用途，以將腫瘤表現的或內源性的IL-8作為標靶。較佳地，該重組IL-8抗體或多價單鏈可變片段(scFv)片段包含一包括選自SEQ ID NO. 1-15, 31-32的一第一胺基酸序列的VH區段，及/或一包括選自SEQ ID NO. 16-30, 33-34的一第二胺基酸序列的VL區段。該重組IL-8抗體或多價單鏈可變片段(scFv)片段可以被配製為藥物組合物，以施予一患有腫瘤的患者以減少該腫瘤的轉移、減少該腫瘤微環境中的免疫抑制或減少Th2調節的免疫反應。
