PROBLEM TO BE SOLVED: To provide a set apparatus of a fruit body guide for mushroom cultivation in which past operation by people can be reasonably mechanized, and that can improve working efficiency.SOLUTION: A set apparatus of a fruit body guide for mushroom cultivation includes: a holding part 15 in which a fruit body guide 10 of steps piling is arranged in a matrix form, is maintained, and is provided to be able to perform movement to a vertical direction relatively two or more holding parts 20 set to hold an upper end edge part 10b of the fruit body guide 10 of the highest step at the same time for the two or more fruit body guides of steps piling a pressing member 30 that touches an upper end 10a of the fruit body guide 10 of a second step from an upper part from an upper part, and presses the fruit body guide 10 of the second step and the fruit body guide 10 of a further lower part so as not to lift when separating to extract the fruit body guide 10 of the highest step and a separation movement mechanism section 50 that moves the holding part 20 in a relatively separating direction of a vertical direction to the holding part 15 of the fruit body guide and the pressing member 30.COPYRIGHT: (C)2014,JPO&INPIT【課題】従来の人手による作業を合理的に機械化することができ、作業効率を向上できるきのこ栽培用子実体ガイドのセット装置を提供すること。【解決手段】段重ねの子実体ガイド10がマトリックス状に配置されて保持され、上下方向へ相対的に移動できるように設けられた子実体ガイドの保持部15と、複数の段重ねの子実体ガイドについて、最上段の子実体ガイド10の上端縁部10bを同時に把持できるように配設された複数の把持部20と、上から二段目の子実体ガイド10の上端10aに上から当接し、最上段の子実体ガイド10を抜き取るように分離する際に二段目の子実体ガイド10及びさらに下段の子実体ガイド10が浮き上がらないように押える押え部材30と、子実体ガイドの保持部15及び押え部材30に対して、把持部20を上下方向の相対的に離れる方向へ移動させる分離動機構部50とを具備する。【選択図】図1