The present invention relates to a composition comprising microstructures for the controlled release of active agents, in particular ozonized oil. The present invention relates, in particular, to a composition comprising microstructures dispersed in an aqueous phase A), wherein said microstructures comprise an oil-in-water (o/w) microemulsion in which an ozonized oil is present, enclosed in a coating comprising a cationic surfactant containing at least one quaternary ammonium group, a polysaccharide and a polysaccharide modified by quaternized ammonium groups. The present invention also relates to a method for the preparation of the composition and its uses.