Song Liu,Yue Feng,Jian Chen,Guocheng Du,Yun Jiao,Jie Ruan,Hongye Cheng,Hui Gao
申请号：
US15189139
公开号：
US09896678B2
申请日:
2016.06.22
申请国别(地区):
US
年份:
2018
代理人:
摘要：
The present invention provides a mutant with enhanced secretion of L-asparaginase and its application, which relates to the field of enzyme engineering. This invention successfully constructed a mutant with enhanced secretion of L-asparaginase through N-terminal deletion on amino acid sequence of L-asparaginase, and then expressed the mutant via a recombinant strain. Compared with the wild L-asparaginase, the secretion ability of L-asparaginase mutant in present invention significantly improves by 3.14 times compared to that of wild type. The recombinant strain that constructed in this prevention has an L-asparaginase yield of 407.6 U/mL and a production efficiency of 9.26 U/(mL/h), which reaches to the highest yield been reported.
