FRYER, BENJAMIN,LANIAUSKAS, DAINA,BLACKMOORE, MARCIA,WANG, HAIYUN,LILOVA, KOSTADINKA,NELSON, SHELLEY,ROSOCHA, ELIZABETH
申请号：
PH12016500782
公开号：
PH12016500782A1
申请日:
2016.04.27
申请国别(地区):
PH
年份:
2016
代理人:
摘要：
The disclosure provides methods of preparing aggregated pluripotent stem cell clusters for differentiation to endoderm progenitor cells, pancreatic endocrine cells, mesoderm cells or ectoderm cells. Specifically, the disclosure provides methods of differentiating pluripotent cells into beta cell, cardiac cell and neuronal cell lineages using suspension clustering. The methods involve preparing the aggregated cell clusters followed by differentiation of these clusters.
