David Frendewey,Gustavo Droguett,Anthony Gagliardi,Junko Kuno,Wojtek Auerbach,David M. Valenzuela
申请号：
US14751807
公开号：
US09902971B2
申请日:
2015.06.26
申请国别(地区):
US
年份:
2018
代理人:
摘要：
Methods and compositions are provided for generating targeted genetic modifications on the Y chromosome or a challenging target locus. Compositions include an in vitro culture comprising an XY pluripotent and/or totipotent animal cell (i.e., XY ES cells or XY iPS cells) having a modification that decreases the level and/or activity of an Sry protein； and, culturing these cells in a medium that promotes development of XY F0 fertile females. Such compositions find use in various methods for making a fertile female XY non-human mammal in an F0 generation.
