A full-color deep-layer imaging system thereof utilizing acousto-optic tomography, including: at least an incident light source, a heterodyne interference system, and a negative display system. Wherein, at least an incident light source emits light to a heterodyne interference system. The heterodyne interferometer system modulates the incident light into a modulated light, and transmits it to a sample tissue for scanning, the sample tissue absorbs the modulated light and produce an acoustic wave, and an oscilloscope is used to display the acoustic wave. Then, a negative display system receives the acoustic wave to determine its travel time from tissue layer, and calculate the deepness of the imaging layer, and restore the image to full color image based on the complimentary images of red, green, blue incident lights.本創作係為一種應用光聲斷層掃描之全彩深層造影系統,其係利用至少一入射光源發出光線至一外差干涉系統;外差干涉系統將入射光源調變為具有聲音頻率之一調變光源後,射出調變光源至一待測組織進行掃描,待測組織吸收光後並產生一超音波,利用一示波器顯示超音波之波形;以及示波器將超音波之波形傳送至一負片顯像系統,判斷超音波之到達時間以計算出待測組織之深度影像,並根據紅、藍、綠入射光源所得負片影像之互補影像將待測組織之深度影像還原至彩色影像。10‧‧‧入射光源122‧‧‧訊號產生器124‧‧‧聲光驅動器126‧‧‧聲光調制器128‧‧‧鏡片組14‧‧‧待測組織16‧‧‧負片顯像系統18‧‧‧超音波探頭20‧‧‧示波器