A cell cryopreservation tool (1) has a cell holding member (2) having a body part (23) and a cell holding part (21) and a tubular accommodation member (3), closed at one end thereof, which is capable of accommodating the cell holding member (2). The cell holding part (21) of the cell holding member (2) has a long and narrow cell attaching and holding portion (22). The cell attaching and holding portion (22) has a heat conductor (25, 26) extended in a longitudinal direction thereof. When the cell holding member (2) is accommodated in the tubular accommodation member (3), the heat conductor is capable of contacting an inner surface of the tubular accommodation member (3).