A living cell cryopreservation tool (1) has a living cell holding member (2) having a body part (23) and a living cell holding part (21) and a tubular accommodation member (3), closed at one end thereof, which is capable of accommodating the living cell holding member (2). The living cell holding part (21) has a long and narrow living cell attaching and holding portion (22). The living cell attaching and holding portion (22) has heat conductors (25, 26) extended in a longitudinal direction thereof and projected from a distal end thereof. The tubular accommodation member has a heat conductive member (32) accommodated inside a distal portion thereof. When the living cell holding member is inserted into the tubular accommodation member from a distal side of each heat conductor thereof, a distal portion (25a, 26a) of each heat conductor contacts the heat conductive member of the tubular accommodation member.