The present invention discloses a micro-fluidic device for separating targets from a sample and a target separating method using the same. The micro-fluidic device includes a filter case, a first capture array and a second capture array. The filter case includes an inlet for introducing a sample containing different kinds of targets, an outlet for discharging the sample and a channel extending between the inlet and the outlet. The first capture array is arranged in an upstream portion of the channel, the first capture array including a plurality of first forward funnels arranged along a direction orthogonal to a flow direction of the sample so as to capture the different kinds of targets. The second capture array is arranged in a downstream portion of the channel, the second capture array including a plurality of second forward funnels arranged along the direction orthogonal to the flow direction of the sample so as to capture the different kinds of targets.
