The present invention discloses a micro-fluidic device for separating targets from a sample. The micro-fluidic device includes a filter cabinet, a first filtering unit and a second filtering unit. The filter cabinet includes a first path and a second path branched from the first path. The first path and the second path are formed within the filter cabinet so that a sample containing different kinds of targets can flow through the first path and the second path. The filter cabinet further includes a introduction hole formed in an upper portion of the filter cabinet to supply the sample into the first path and a discharge hole formed in a lower portion of the filter cabinet to discharge the sample from the first path and the second path. The first filtering unit is installed in an upstream portion of the first path to filter the different kinds of targets from the sample, the first filtering unit configured to guide the different kinds of targets toward the second path. The second filtering unit is installed in the second path to receive the different kinds of targets from the first filtering unit and to filter the different kinds of targets on a size-by-size basis.
