An apparatus (1), for enzymatic inactivation of puree, or juice, from vegetable or animal food, comprises a treatment section (10) of food (100) of vegetable origin obtaining a treated product. The treated product exiting from the treatment section (10) is then directed, for example through a duct (13), towards an extraction section (20). More precisely, the treated product enters the extraction section (20) at a temperature T0, for example set between about −25° C. and about +30° C. The apparatus (1) is further provided with a storage and recirculation section (30), comprising, in particular, an enzymatic inactivation circuit crossed by a hot product at a temperature T2, higher than the enzymatic inactivation temperature T*, normally is about 85° C.-90° C., of the main product. The main product present in the extraction section (20) is struck, near the sieve (22), or directly on its surface, by a flow of hot product coming from the enzymatic inactivation section (30). The mixture so obtained in the extraction section (20) comprises at least one part of inactivated product and is then discharged through an outlet (41).