David E. Anderson;Hoang-Thanh Le;Francisco Diaz-Mitoma
发明人:
David E. Anderson,Francisco Diaz-Mitoma,Hoang-Thanh Le
申请号：
US13377371
公开号：
US20120177683A1
申请日:
2010.07.06
申请国别(地区):
US
年份:
2012
代理人:
摘要：
The present disclosure provides methods for preparing vesicles. In general, these methods include steps of providing a lyophilized lipid product and rehydrating the lyophilized lipid product with an aqueous solution comprising an antigen such that antigen-containing vesicles are formed. The lyophilized lipid product is prepared by dissolving vesicle-forming lipids in a polar-protic water-miscible organic solvent to produce a lipid solution and then lyophilizing the lipid solution. The present disclosure also provides antigen-containing vesicle formulations prepared using these methods. The present disclosure also provides kits that include a lyophilized lipid product in a first container and an aqueous solution comprising an antigen in a second container.
