There is provided a test kit that can be used to test a test sample prepared by processing a specimen using two kinds of pharmaceutical agents. The test kit is compact, capable of readily and reliably preparing the test sample, and improved in user- friendliness. The test kit comprises a chamber (1), an inner chamber (2), a sealing member (3), and a specimen collecting tool (4). An annular groove (21) is formed in a peripheral portion of an outer surface on a bottom portion (20) of an inner chamber (2), and a thin film portion (23) constituting a side surfaces (21a) and (21b) facing each other constituting the groove (22) connects the bottom portion (20) with the other portions of the inner chamber 2. A specimen collecting tool (4) presses a predetermined position Q in the peripheral portion of the bottom portion, stretching the thin film portion. When the thin film portion thus stretches, tiling the bottom portion, side surfaces (21a) and (21b) constituting the groove (21) abut each other at a predefined position P in the peripheral direction of the bottom portion, and the stretch of the thin film portion (23) is restricted nearby. When the pressing force is further applied to the predefined position Q by the specimen collecting tool, the thin film portion (23) further stretches near the position Q to finally break, and the specimen collecting tool penetrates through the bottom portion of the inner chamber.La présente invention concerne un kit de test, qui peut être utilisé pour tester un échantillon test préparé par traitement dun prélèvement au moyen de deux types dagents pharmaceutiques. Le kit de test est compact, permet de préparer facilement et de manière fiable léchantillon test, et est amélioré du point de vue de sa praticité. Le kit de test comprend une chambre (1), une chambre interne (2), un élément détanchéité (3) et un instrument (4) de récupération de prélèvement. Une rainure annulaire (21) est formée dans une partie périphérique dune surface externe,
