DAI, YONG,WANG, YONG,JIN, SHENGJIN,MESHULAM, DEBORAH H,AMPHLETT, GODFREY W
申请号：
NZ59543006
公开号：
NZ595430A
申请日:
2006.08.14
申请国别(地区):
NZ
年份:
2013
代理人:
摘要：
595430 Disclosed is a process for preparing an antibody-cytotoxic agent conjugate comprising the steps of: (a) contacting an antibody with a bifunctional crosslinking reagent to covalently attach a linker to the antibody and thereby prepare a first mixture comprising antibodies having linkers bound thereto, (b) conjugating a cytotoxic agent to the antibodies having linkers bound thereto in the first mixture by reacting the antibody having linkers bound thereto with a cytotoxic agent in a solution having a pH of about 4 to about 9 to prepare a second mixture comprising (i) antibody chemically coupled through the linker to the cytotoxic agent, (ii) free cytotoxic agent, and (iii) reaction by-products produced during step (b), and (c) subjecting the second mixture to tangential flow filtration, selective precipitation, adsorptive filtration, adsorptive chromatography, or a combination thereof, to purify the antibodies chemically coupled through the linkers to the cytotoxic agent from the other components of the second mixture and thereby prepare a purified second mixture of antibodies chemically coupled through the linkers to the cytotoxic agent wherein the first mixture is not subjected to purification.
