Provided is an estimating method for electrophysiologically estimating the numbness and spontaneous pain, which can not be estimated by animal behavior experiment in the past, by using the firing pattern of the animal peripheral nerve action potential to estimate numbness or spontaneous pain.本發明之目的係提供可藉由電生理學方式評估以往無法以動物行為實驗評估之麻木感、自發性疼痛之評估方法。本發明之解決手段係使用動物末梢神經所產生之動作電位之放電模式以評估麻木或自發性疼痛。該代表圖無元件符號及其代表之意義。