An improved method for the extraction of glycosides from pineapple peel is provided. The peel of Pineapple (Ananas comosus) fruit, an agro-waste, is extracted for glycosides with 4 times volume of isopropyl alcohol, at ambient temperature with stirring, for 3 hours each at thrice. The solvent is distilled out under vacuum at 60°C to 80 °C and the un-distilled mass is added with 4 times acetone, LR (v/v). The resulting precipitate obtained is vacuum dried at 60 to 80°C. An off creamy hygroscopic powder is obtained, which has glycosidal test on developing TLC confirming the positive test for glycosides. The glycoside has found application in natural detergent industry and in cosmetics.