Andrew Martin Pickett,Robin Andrew Quirk,Richard Melville France,Lisa Anne Riccalton-Banks
申请号：
US10545009
公开号：
US08877456B2
申请日:
2004.02.20
申请国别(地区):
US
年份:
2014
代理人:
摘要：
The invention relates to a method for determining the quantity of pre-synaptic neuromuscular blocking substance (notably botulinum toxin) contained in a sample. In one aspects, the method comprises the following steps: (i) determining the minimum voltage Vm needed to induce the contraction of muscle tissue, said muscle tissue being connected to an electrical stimulator through a motor nerve and preferably immersed in an oxygenated physiological buffer containing glucose (ii) adding the sample containing the pre-synaptic neuromuscular blocking substance (iii) electrically stimulating, at a voltage at least equal to Vm, the muscle tissue at certain time intervals (iv) comparing the effect induced by the sample to the effect induced by a reference substance and thereby determining the quantity of the pre-synaptic neuromuscular blocking substance in the sample.
