Disclosed are a trifluoroacethydrazide compound as well as preparation and application thereof. The compound has a structure represented by general formula (I). The compound has multiple mechanisms of action, comprising inhibition of glutamate excitotoxicity, activation of MEF2 transcriptional activity, and the effect of scavenging free radicals and promoting neural differentiation, and thus has a desirable protective effect on neurocytes and can alleviate memory impairment of a scopolamine-induced mouse model. The compound can be used for preparing a medicament having a cell protective effect, which is used for preventing or treating diseases related to glutamate excitotoxicity, MEF2 imbalance or overproduction of free radicals, e.g., diseases related to neurodegeneration comprising senile dementia, Parkinson's disease, cerebral stroke and the like, and diseases related to free radicals such as heart disease, myocardial ischemia, diabetes and other cardiovascular and cerebrovascular diseases.
