A multi-layer antimicrobial wound dressing includes a wound-contacting layer and an antimicrobial layer. The wound contacting layer includes a biocompatible fibrous matrix that is made of an anionic polymer, and the antimicrobial layer is made of a cationic polymer that is connected to the wound-contacting layer via anionic-cationic interaction between the anionic polymer of the fibrous matrix and the cationic polymer of the antimicrobial layer.一種高吸水性抗菌敷材包含一高吸水性纖維基材及一抗菌背層，其中，高吸水性纖維基材具有多數條纖維，且該等纖維係由一帶負電之聚合物所構成，而抗菌背層係由一帶正電之聚合物所構成。該抗菌背層係藉由該帶正電之聚合物與該帶負電之聚合物間的正負電相吸作用而與該高分子纖維基材的上層部相結合，且該帶正電之聚合物係附著於該高分子纖維基材的上層部的纖維表面上。本發明高吸水性抗菌敷材吸水性佳、具抗菌性且天然而具環保性。
