A blood separation method is provided. The blood separation method includes the following steps. Firstly, a whole blood is placed in a container having a separating gel, which includes a silicon-containing polymer, and a specific gravity of the separating gel is between 1.030 and 1.093. Then, a centrifugation is performed at a first effective rotation speed to divide the whole blood into a red blood cell layer located below the separating gel, a buffy coat layer located above the separating gel, and a plasma layer located above the buffy coat layer. The buffy coat layer and the plasma layer are then mixed to obtain a platelet-rich plasma.
