A method for blood separation, where different blood fractions are obtained, specifically erythrocytes, leukocyte layer and blood plasma, where blood is introduced into a separation vessel and then centrifuged in different fluidically connected sections of the separation vessel arranged one on top of the other, specifically an upper section that receives the plasma, a central section for the reception of the leukocyte layer and a lower section for the reception of the erythrocytes, where from the hematocrit value of the blood supplied, the future packaging volume of erythrocytes to be centrifuged, where the capacity of the lower section adapts to the expected packaging volume of erythrocytes after centrifugation such that the phase limit formed between the leukocyte layer and erythrocytes during centrifugation is position in a region of the central section of the separation vessel that is adjacent to the lower section, and where the amount of blood supplied is introduced into the separation vessel in an exact volume, taking into account the expected packaging volume of the erythrocytes, characterized in that after centrifugation, the lower section (2) is separated from the central section (3), and because the central section (3) is part of a freezer container (12) which, during centrifugation, is separated from the other region of the freezer container (12) , and because after centrifugation, the connection to the previously separated region of the freezer vessel (12) occurs and the leukocyte layer passes, at least in part, to the entire freezer vessel (12).Un método para la separación de sangre, donde se obtienen las diferentes fracciones de sangre, específicamente eritrocitos, capa leucocitaria y plasma sanguíneo, donde la sangre se introduce en un recipiente de separación y entonces se centrifuga en diferentes secciones conectadas fluídicamente del recipiente de separación dispuestas una encima de la otra, específicamente una sección superior que recibe el plasma