РЕКОМБИНАНТНАЯ ПСЕВДОАДЕНОВИРУСНАЯ ЧАСТИЦА НА ОСНОВЕ ГЕНОМА АДЕНОВИРУСА ЧЕЛОВЕКА 5 СЕРОТИПА, ПРОДУЦИРУЮЩАЯ ГЕМАГГЛЮТИНИН ВИРУСА ГРИППА ШТАММА A/Brisbane/59/2007(H1N1), СПОСОБ ЕЕ ИСПОЛЬЗОВАНИЯ ДЛЯ ИНДУКЦИИ СПЕЦИФИЧЕСКОГО ИММУНИТЕТА К ВИРУСУ ГРИППА А СУБТИПОВ Н1 И Н5
Общество с ограниченной ответственностью "НТфарма" (RU)
发明人:
Атауллаханов Рустам Равшанович (RU)
申请号：
RU2012158146/10
公开号：
RU2012158146A
申请日:
2012.12.29
申请国别(地区):
RU
年份:
2014
代理人:
摘要：
1. Recombinant psevdoadenovirusnaya particle on the basis of human adenovirus serotype 5 genome, containing an expression cassette insert influenza hemagglutinin gene, wherein the gene chtov as influenza virus hemagglutinin gene is used hemagglutinin influenza virus strain A / Brisbane / 59/2007 (H1N1) with pre optimized for expression in human cells a nucleotide sequence with increased gene expression software hemagglutinin influenza a / Brisbane / 59/2007 (H1 N1) .2. Recombinant psevdoadenovirusnaya particle according to claim 1, characterized in that the nucleotide sequence of the optimized gene hemagglutinin influenza virus strain A / Brisbane / 59/2007 (H1N1) is SEQ ID NO: 2.3. Recombinant psevdoadenovirusnaya particle according to claim 1, characterized in that the hemagglutinin gene of influenza strain A / Brisbane / 59/2007 (H1N1) with optimized nucleotide sequence is cloned into an expression cassette under the control of a promoter and signal comprising poliadenilirovaniya.4. Recombinant psevdoadenovirusnaya particle according to claim 3, characterized in that the promoter is a promoter tsitomegalovirusa.5. Recombinant psevdoadenovirusnaya particle according to claim 3, characterized in that the polyadenylation signal is SV40.6. Recombinant psevdoadenovirusnaya particle according to claim 1, characterized in that the expression cassette is located in the E1 deletion of adenovirus 5 genome serotipa.7. A method of using rekombinantnoi psevdoadenovirusnoy particles according to claim 1 based on the human adenovirus serotype 5 genome, producing virus hemagglutinin influenza A / Brisbane / 59/2007 (H1N1) by its administration in an effective amount for induction1. Рекомбинантная псевдоаденовирусная частица на основе генома аденовируса человека 5 серотипа, содержащая экспрессирующую кассету со вставкой гена гемагглютинина вируса гриппа,отличающаяся тем, чтов качестве гена гемагглютинина вируса гриппа используют ген гемагглютинина вируса гриппа штамма A/Brisbane/
