Provided is a fluorescence observation apparatus including: a fluorescence image acquisition section and a reference image acquisition section that acquire a fluorescence image and a reference image of a subject, respectively a division image generation section that generates a division image by dividing an image based on the fluorescence image by an image based on the reference image a display section that displays a corrected fluorescence image based on the division image a correction processing section that applies correction processing to at least one of the reference image and the fluorescence image and/or to the division image prior to the generation of the division image or prior to the display of the corrected fluorescence image an observation condition determination section that determines observation conditions of the subject and a correction condition setting section that sets parameters regarding the correction processing according to the observation conditions.
