PROBLEM TO BE SOLVED: To provide a sensor element which sufficiently fixes a mediator participating in electron transfer between an enzyme and a working electrode onto the working electrode and is hardly diffused in a sensor, and a measuring device comprising the sensor element.SOLUTION: A sensor element comprises: a base section (substrate) a working electrode provided on the base section a detection layer provided on the working electrode and a counter electrode where electrons move between the counter electrode and the working electrode. The detection layer includes: an enzyme acting on a target substance contained in liquid a water-soluble mediator mediating electron transfer between the enzyme and the working electrode and a hydrophilicity host material crosslinked to the mediator.SELECTED DRAWING: NoneCOPYRIGHT: (C)2018,JPO&INPIT【課題】酵素と作用電極との間の電子移動に関与するメディエーターを作用電極上に十分に固定して、センサー内で拡散しにくいセンサー素子及びそのセンサー素子備えた測定装置を提供する。【解決手段】本発明に係るセンサー素子は、基部(基板)と、基部に設けられた作用電極と、作用電極に設けられた検知層と、作用電極との間で電子が移動する対電極と、を備え、検知層は、液体に含まれる標的物質に作用する酵素と、酵素と作用電極との間の電子移動を媒介する水溶性のメディエーターと、メディエーターに架橋結合した親水性のホスト材料と、を含む。【選択図】なし