PROBLEM TO BE SOLVED: To provide a detection element and a measurement device capable of accurately measuring density of a material to be measured even in a wide density range, and a reliable agent feeding device, a manufacturing method for the detection element capable of efficiently manufacturing the detection element.SOLUTION: A detection element includes a first working electrode 3111 provided with a first sensing layer 3201 containing an enzyme that reacts with a material to be measured, a second working electrode 3112 provided with a second sensing layer 3202 with more enzyme content than that of the first sensing layer, and a counter electrode 312. A film thickness of the second sensing layer is preferably larger than a film thickness of the first sensing layer. The detection element is further preferably provided with a third working electrode 3113, and the third working electrode is further preferably provided with an enzyme-free layer that does not contain the enzyme.SELECTED DRAWING: Figure 6【課題】広い濃度範囲でも測定対象物質の濃度を高精度に測定可能な検出素子および測定装置、信頼性の高い薬剤供給装置、ならびに、前記検出素子を効率よく製造し得る検出素子の製造方法を提供する。【解決手段】測定対象物質と反応する酵素を含有した第１感知層３２０１が設けられた第１作用電極３１１１と、酵素の含有量が第１感知層より多い第２感知層３２０２が設けられた第２作用電極３１１２と、対電極３１２と、を備えることを特徴とする検出素子。また、第２感知層の膜厚は、前記第１感知層の膜厚より厚いことが好ましい。また、第３作用電極３１１３をさらに備えることが好ましく、第３作用電極には、酵素を含有しない無酵素層がさらに設けられていることが好ましい。【選択図】図６
