John J. Donnelly,Kimberly S. Denis-Mize,Gary S. Ott
申请号：
US12755266
公开号：
US08097243B2
申请日:
2010.04.06
申请国别(地区):
US
年份:
2012
代理人:
摘要：
The present invention provides an effective method for the transfection of dendritic cells by non-viral methods. The present invention provides this benefit by incubating dendritic cells and a specified transfection agent. The transfection agent comprises a polynucleotide and microparticles, with the microparticles being comprised of biodegradable polymer and cationic detergent. The dendritic cells and transfection agent are incubated for a time sufficient to transfect the dendritic cells with the polynucleotide.
