The present invention relates to cold-adapted temperature sensitive Enterovirus 71 strains, particularly to the cold-adapted temperature sensitive Enterovirus 71 strains EV71:TLLβP20 and EV71:TLLαP20. The present invention also relates to processes of developing the cold-adapted temperature sensitive virus strains.本發明係關於適於冷之腸病毒71溫度敏感株,特定言之適於冷之腸病毒71溫度敏感株EV71:TLLβP20及EV71:TLLαP20。本發明亦關於發展該等適於冷之溫度敏感病毒株之方法。