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METHOD AND DESIGN FOR SCBV SYNTHETIC BIDIRECTIONAL VEGETABLE PROMOTOR
专利权人:
ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи (US)
发明人:
КУМАР Сандип (US),АЛАБЕД Диаа (US),БЕННЕТТ Сара (US),ГУПТА Манджу (US),ДЖЭЙН Сьюзан (US),РАЙТ Терри (US)
申请号:
RU2014131447
公开号:
RU2014131447A
申请日:
2012.11.12
申请国别(地区):
RU
年份:
2016
代理人:
摘要:
1. A synthetic polynucleotide containing an element of the minimal core promoter from the ubiquitin-1 gene of Zea mays or Zea luxurians and the functional nucleotide sequence of the promoter from the promoter of the bacillary sugarcane virus. 2. The synthetic polynucleotide of claim 1, wherein the minimal core promoter element forms a polynucleotide sequence that is at least 75% identical to SEQ ID NO: 1 or its complementary strand. The synthetic polynucleotide of claim 1, wherein the minimal core promoter element forms SEQ ID NO: 1 or a complementary strand thereof. The synthetic polynucleotide according to claim 1, further comprising an exon from the ubiquitin-1 gene and an intron from the ubiquitin-1.5 gene. The synthetic polynucleotide according to claim 1, further comprising an intron from the alcohol dehydrogenase gene. The synthetic polynucleotide according to claim 1, containing an upstream regulatory sequence from the promoter of the bacillary sugarcane virus. The synthetic polynucleotide according to claim 5, wherein the functional nucleotide sequence of the promoter from the promoter of the bacillary sugarcane virus and the alcohol dehydrogenase gene contains SEQ ID NO: 6 or its complementary chain. The synthetic polynucleotide according to claim 1, further comprising an element selected from the group consisting of the upstream regulatory sequence (URS), enhancer element, exon, intron, transcription start site, TATA box, heat shock consensus element, nucleotide start sequence and / or termination of translation and their combinations. 9. The synthetic polynucleotide of claim 1, further comprising a nucleot of interest1. Синтетический полинуклеотид, содержащий элемент минимального корового промотора из гена убиквитина-1 Zea mays или Zea luxurians и функциональную нуклеотидную последовательность промотора из промотора бациллярного вируса сахарного тростника.2. Синтетический полинуклеотид по п. 1, где элемент минимального корового промотора образует полинук
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