#$%^&*AU2020102109A420201015.pdf#####ABSTRACT This invention is related to the field of medicine, particularly, related to an extracting and purifying technology of trehalose from Cordyceps militaris and trehalose product and application thereof. The method for extracting and purifying trehalose from Cordyceps militaris comprises the following steps: Mixing the raw material of Cordyceps militaris with alcohol solution in a mass ratio of 1:10-50, extracting the mixture with ultrasound, filtering the extract of Cordyceps militaris, Crystallizing the extract to obtain trehalose crystal, purifying the crystal obtained. Extracting and purifying trehalose from Cordyceps militaris is to make full use of this component contains in the Cordyceps militaris, so that it can realize targeted use of its function, and also improve its use efficiency.