CHIOU, SHIH HWA,邱士华,邱士華,PERNG, CHERNG KANG,彭成康,LIN, HAN TZO,林翰佐,YANG, YI PING,杨逸萍,楊逸萍
申请号：
TW096143658
公开号：
TWI374743B
申请日:
2007.11.19
申请国别(地区):
TW
年份:
2012
代理人:
摘要：
A composite comprising a stem cell； a biodegradable layer, which can provide an environment for the stem cell to grow and to differentiate, and； a N-isopropylacrylamide (NIPAAm), which can polymerize with the biodegradable layer and possess the temperature-responsive character for easy stripping. This invention also provides a method of treating a subject with a skin defect by covering the composite of the present invention on the skin defect of the subject in need of such treatment. Furthermore, using this composite with different growth factors, stem cells can be induced to differentiate into skin-related, neuronal cells, neuron, and insulin-positive cells in biodegradable scaffolds as well as transplanted graft. Finally, this invention also provides a quick and convenient method of monitoring cell growth or tissue engineering in an animal.一種層合物，包含幹細胞、提供幹細胞生長及分化環境之生物可分解層、可和生物可分解層一起聚合並具有感溫性而易於剝離之異丙基丙烯醯胺。本發明亦提供一種治療皮膚或組織缺陷之方法，係藉由覆蓋本發明之組合物於皮膚或組織缺陷需要治療之處。更進一步地，本發明之組合物可結合不同生長激素，誘導在生物可分解支架及移植組織中之幹細胞分化成皮膚相關細胞、神經細胞及胰島素陽性反應細胞。本發明亦進而提供迅速方便之方法，來監測動物體內之細胞生長或組織工程。
