LAGLIDADG homing endonuclease variant with novel substrate specificity and use thereof

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专利权人:

セレクティス

发明人:

モントヤ，ギレルモ,ブランコ，フランシスコ,プリエト，ヘスス,モントヤ,ギレルモ,ブランコ,フランシスコ,プリエト,ヘスス

申请号：

JP2009549850

公开号：

JP5524627B2

申请日:

2007.02.19

申请国别(地区):

JP

年份:

2014

代理人:

摘要：

A LAGLIDADG homing endonuclease variant having novel substrate specificity, said variant being obtainable by a method comprising: (a) the mutation of at least one amino acid residue of the final C-terminal loop of a parent LAGLIDADG homing endonuclease, with the exclusion of the threonine 140 of I-CreI, b) the selection and/or screening of the variants from step (a) having a pattern of cleaved DNA targets that is different from that of the parent LAGLIDADG homing endonuclease.

来源网站：

中国工程科技知识中心

来源网址：

http://www.ckcest.cn/home/