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USE OF THE RD29 PROMOTER OR FRAGMENTS THEREOF FOR STRESS-INDUCIBLE EXPRESSION OF TRANSGENES IN COTTON.
专利权人:
BAYER CROPSCIENCE NV
发明人:
PIEN, Stéphane,DEN BOER, Bart
申请号:
MX2013008507
公开号:
MX356630B
申请日:
2012.01.24
申请国别(地区):
MX
年份:
2018
代理人:
摘要:
In one aspect, the present application discloses a chimeric gene comprising (a) a first nucleic acid sequence comprising at least 400 consecutive nucleotides of SEQ ID NO: 1 or SEQ ID NO: 2 or a nucleic acid sequence having at least 80% sequence identity thereto any of which confers stress inducibility on said chimeric gene (b) a second nucleic acid sequence encoding an expression product of interest, which is involved in the response of a cotton plant to stress and optionally (c) a transcription termination and polyadenylation sequence. In another aspect, the application discloses a cotton plant cell comprising (a) a chimeric gene comprising a first nucleic acid sequence comprising at least 400 consecutive nucleotides of SEQ ID NO: 1 or SEQ ID NO: 2 or a nucleic acid sequence having at least 80 sequence identity thereto any of which confers stress inducibility on said chimeric gene (b) a second nucleic acid sequence encoding an expression product of interest and optionally (c) a transcription termination and polyadenylation sequence. In addition, the present application discloses a cotton plant, a method of expressing a transgene in cotton under stress conditions, a method of producing a cotton plant, a method of detecting the expression of a transgene under stress conditions and a method for modulating the resistance of a cotton plant to stress as characterized in the claims.La presente invención se refiere a una célula de planta de algodón, caracterizada porque comprende un gen quimérico que comprende (a) una primera secuencia de ácido nucleico que comprende la secuencia de nucleótidos de SEQ ID NO: 1 o SEQ ID NO: 2 que confiere inducibilidad de estrés sobre dicho gen quimérico (b) una segunda secuencia de ácido nucleico que codifica un producto de expresión de interés y (c) una secuencia de terminación y poliadenilación en donde dicho producto de expresión de interés es una proteína o péptido seleccionado de PNC1 (pirazinamidasa/inocitinamidasa 1), NMA1 (ácido n
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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