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Use of the rd29 promoter or fragments thereof for stress-inducible expression of transgenes in cotton
专利权人:
Bayer CropScience N.V.
发明人:
Pien, Stephane,Den Boer, Bart
申请号:
AU2012210618
公开号:
AU2012210618A1
申请日:
2012.01.24
申请国别(地区):
AU
年份:
2013
代理人:
摘要:
In one aspect, the present application discloses a chimeric gene comprising (a) a first nucleic acid sequence comprising at least 400 consecutive nucleotides of SEQ ID NO: 1 or SEQ ID NO: 2 or a nucleic acid sequence having at least 80% sequence identity thereto any of which confers stress inducibility on said chimeric gene (b) a second nucleic acid sequence encoding an expression product of interest, which is involved in the response of a cotton plant to stress and optionally (c) a transcription termination and polyadenylation sequence. In another aspect, the application discloses a cotton plant cell comprising (a) a chimeric gene comprising a first nucleic acid sequence comprising at least 400 consecutive nucleotides of SEQ ID NO: 1 or SEQ ID NO: 2 or a nucleic acid sequence having at least 80 sequence identity thereto any of which confers stress inducibility on said chimeric gene (b) a second nucleic acid sequence encoding an expression product of interest and optionally (c) a transcription termination and polyadenylation sequence. In addition, the present application discloses a cotton plant, a method of expressing a transgene in cotton under stress conditions, a method of producing a cotton plant, a method of detecting the expression of a transgene under stress conditions and a method for modulating the resistance of a cotton plant to stress as characterized in the claims.
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