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Preparāta "Mikrocit" iegūšanas veids
专利权人:
MIŠKE Irina
发明人:
MIKLAŠĒVIČS, Edvīns,MIŠKE, Irina,TEVELEVA, Marina
申请号:
LV920509
公开号:
LV15531A
申请日:
1992.12.28
申请国别(地区):
LV
年份:
2020
代理人:
摘要:
Preparation "Mikrocits" exhibits cytokinine activity and it can be widely used as unsubstitutable component for producing culture medium for different tissue and cell growing. Known method of producing preparation "Mikrocits" according to a.c. of the USSR 1304402 is modified in such way as the bacteria before plating on solid agar medium surface are kept 1,5-5 hours at temperature 18-200C. Culture medium additionally contains sodium acetate as a source of carbon and all medium components are used is the following ratio (g/l): potassium dihydrogenphosphate 1,0-1,2; ammonium sulphate 1,0-1,5; magnesium sulphate 0,1-0,5; glycerol 6,4-7,5; succinic acid 0,8-1,5; ferrous chloride 0,001-0,002; microelements by Fyodorov 0,065-0,075 and distilled water up to 1 litre.Preperâtam "Mikrocits" piemìt citokinìnu aktivitâte un to var plaøi pielietot kâ neaizvietojamu komponentu barotðu pagatavoøanai augu audu un øúnu kultúru audzèøanai daýâdiem mèræiem. Ar mèræi palielinât galaprodukta iznâkumu un aktivitâti, tiek piedâvâts zinâmo "Mikrocita" iegúøanas paðèmienu (PSRS a.a. Nr. 1304402) modificèt tâdèjâdi, ka baktèrijas pirms uzsèøanas uz cietâs agarizètâs barotnes iztur 1,5-5 stundas 18-20OC temperatúrâ, barotnè papildus kâ oglekîa avotu pievieno nâtrija acetâtu un vides komponentus izmanto øâdâs attiecìbâs, g/l:kâlija dihidrogènfosfâts - 1,0-1,2;amonija sulfâts - 1,0-1,5;magnija sulfâts - 0,1-0,5;glicerìns - 6,4-7,5;dzintarskâbe - 0,8-1,5;dzelzs hlorìds - 0,001-0,002;mikroelementi pèc Fjodorova - 0,065-0,075;destilèts údens - lìdz 1 l.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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