LUGOVSKOY, Alexey A.,TAYLOR, Frederick R.,MCLACHLAN, Karen
申请号：
ES11730120
公开号：
ES2633105T3
申请日:
2011.04.15
申请国别(地区):
ES
年份:
2017
代理人:
摘要：
A recombinant antibody molecule or a fragment thereof that binds to α4 comprising a variable heavy chain and a variable light chain, in which: (a) the variable heavy chain comprises the sequence of the variable heavy chain framework of IGHV1-f and the CDRs of the VH chain of the murine antibody HP1 / 2, in which CDR1 comprises the sequence GFNIKDTYM, CDR2 comprises the sequence RIDPASGDTKYDPKFQV and CDR3 comprises the sequence GMWVSTGYALDF； and (b) the variable light chain comprises the sequence of the framework of the variable light chain of IGKV4-1 and the CDRs of the VL chain of the murine antibody HP1 / 2, in which CDR1 comprises the sequence KASQSVTNDVA, CDR2 comprises the sequence YASNRYT and CDR3 comprises the sequence QQDYSSPYT, in which: (i) amino acid residues in positions 1, 67 and 87 of the framework of the variable light chain according to the Kabat numbering scheme are substituted with non-human residues, in which the non-human residue at position 1 of the framework is serine (S), the non-human residue at position 67 of the framework is tyrosine (Y) and the non-human residue at position 87 of the framework is phenylalanine (F)； and (ii) the amino acid residues at positions 24 and 94 of the framework of the variable heavy chain according to the Kabat numbering scheme are substituted with non-human residues, in which the non-human residue at position 24 of the framework is alanine ( A) and the non-human residue at position 94 of the framework is aspartic acid (D)； and wherein the recombinant antibody molecule or fragment thereof that binds to α4 binds to VLA-4.Una molécula de anticuerpo recombinante o un fragmento de la misma que se une a α4 que comprende una cadena pesada variable y una cadena ligera variable, en la que: (a) la cadena pesada variable comprende la secuencia del armazón de la cadena pesada variable de IGHV1-f y las CDR de la cadena VH del anticuerpo murino HP1/2, en la que CDR1 comprende la secuencia GFNIKDTYM, CDR2 comprende la secuencia
