The invention provides a preparation method of haematococcus pluvialis extracts, a test method and application thereof. The preparation method of the haematococcus pluvialis extracts provided by the invention is a supercritical fluid extraction method using CO2 as extraction media. In the haematococcus pluvialis extracts prepared according to the method, the content of the astaxanthin can reach 2to 20 percent (w/w). The method provided by the invention has the characteristics of simple operation, low energy consumption, controllable quality and the like, the extracts also comprise various nutrient contents necessary for human bodies besides the functional ingredient of the astaxanthin, the haematococcus pluvialis extracts with high quality can be produced, and the raw material requirements required by medicine, health care food and ordinary food production can be met.
